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¡á¡á¡á Á¦Ç°Á¤º¸/½Ã¾à·ù/ Expression Vectors / INVIVOGEN/Multigenic Plasmid-pVITRO2

Ç° ¸ñ ¸í Multigenic Plasmid-pVITRO2  
¿ë    µµ Create gene combinations in a single plasmid        
±¸¼º¼ººÐ @ Æ÷À¯µ¿¹° ¼¼Æ÷¿¡¼­ 2°³ÀÇ À¯ÀüÀÚ¸¦ ¾ÈÁ¤ÀûÀ¸·Î co-expression 
- 2°³ÀÇ °­·ÂÇÑ promoter 
- SV40 & CMV enhancers 
- ±¸Á¦¿ª ¹ÙÀÌ·¯½º (FMDV) IRES(internal ribosome entry site) 
- °³¼±µÈ hygromycin selection 
- 2°³ÀÇ multiple cloning sites ¶Ç´Â 2°³ÀÇ reporter genes 
- Multigenic plasmid, 2°³ transcription units (TU) 
-  Human ferritin promoters: FerL (light) & FerH (heavy) chain
- Ferritin: FerL°ú FerH chainÀ¸·Î ±¸¼ºµÈ ´Ü¹éÁú, ³ÐÀº ¹üÁÖÀÇ cell types¿¡¼­ ¹ßÇö, Iron regulation¿¡ ÀÌ¿ë
- Iron regulation Á¦°Å: FerH¿Í FerLÀÇ 5¡¯UTRÀ» mouse & chimpanzee EF1¥á genesÀÇ 5¡¯UTR·Î ±³Ã¼
- CMV promoterº¸´Ù ´õ °­ÇÏ°í ÀÏÁ¤ÇÑ activity¸¦ º¸¿©ÁÜ


@ pVITRO 2 backbone 
First Transcription Unit     Second Transcription Unit 
Promoter    Human FerH             Human FerL 
Enhancer    SV40                     Human CMV              
Gene            GFP (MCS)             LacZ (MCS) 
IRES            FMDV
Selection   HygroR        
º¸    °ü -20µµ        
À¯È¿±â°ü 1³â        

NO.CAT PRODUCT DESCRIPTION UNIT SIZE ÁÖ¹®¿äû
pvitro2-gfplacz @ pVITRO2-GFP/LacZ ORF(open reading frame)À» ½±°Ô cloningÇÏ¿© control vector·Î ÀÌ¿ëÇÒ ¼ö ÀÖÀ½ 20ug °³
pvitro2-mcs @ pVITRO2-mcs(multiple cloning site) cDNAsÀÇ Æí¸®ÇÑ cloning, 2°³ÀÇ cDNA cloningÀ» À§ÇÑ MCS, ¸¹Àº restriction sites°¡ ÀÖ¾î ligation¿¡ ÀûÇÕÇÔ 20ug °³

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