|
Ç° ¸ñ ¸í |
Multigenic Plasmid-pVITRO2 |
¿ë µµ |
Create gene combinations in a single plasmid |
±¸¼º¼ººÐ |
@ Æ÷À¯µ¿¹° ¼¼Æ÷¿¡¼ 2°³ÀÇ À¯ÀüÀÚ¸¦ ¾ÈÁ¤ÀûÀ¸·Î co-expression
- 2°³ÀÇ °·ÂÇÑ promoter
- SV40 & CMV enhancers
- ±¸Á¦¿ª ¹ÙÀÌ·¯½º (FMDV) IRES(internal ribosome entry site)
- °³¼±µÈ hygromycin selection
- 2°³ÀÇ multiple cloning sites ¶Ç´Â 2°³ÀÇ reporter genes
- Multigenic plasmid, 2°³ transcription units (TU)
- Human ferritin promoters: FerL (light) & FerH (heavy) chain
- Ferritin: FerL°ú FerH chainÀ¸·Î ±¸¼ºµÈ ´Ü¹éÁú, ³ÐÀº ¹üÁÖÀÇ cell types¿¡¼ ¹ßÇö, Iron regulation¿¡ ÀÌ¿ë
- Iron regulation Á¦°Å: FerH¿Í FerLÀÇ 5¡¯UTRÀ» mouse & chimpanzee EF1¥á genesÀÇ 5¡¯UTR·Î ±³Ã¼
- CMV promoterº¸´Ù ´õ °ÇÏ°í ÀÏÁ¤ÇÑ activity¸¦ º¸¿©ÁÜ
@ pVITRO 2 backbone
First Transcription Unit Second Transcription Unit
Promoter Human FerH Human FerL
Enhancer SV40 Human CMV
Gene GFP (MCS) LacZ (MCS)
IRES FMDV
Selection HygroR |
º¸ °ü |
-20µµ |
À¯È¿±â°ü |
1³â |
|
Copyright© 2001 Daeil Science Co.,Ltd. All
rights reserved.
|
|
|