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Promoter-pDRIVE plasmid |
¿ë µµ |
¿øÇÏ´Â promoter¸¦ ´Ù¸¥ vector¿¡ »ðÀÔ |
±¸¼º¼ººÐ |
* Transient transfection ½ÇÇè½Ã¿¡ ¿©·¯ °¡ÁöÀÇ ´Ù¸¥ promoterÀÇ È°¼ºÀ» ºñ±³ÇÒ ¼ö ÀÖÀ½
* °¢°¢ÀÇ promoter´Â ¿ÏÀüÈ÷ sequenceµÇ¾î ÀÖ°í ¿©·¯ ´Ù¸¥ ¼¼Æ÷ÁÖ¿¡¼ ½ÇÇèÀÌ µÇ¾úÀ½
* Native and composite promoter
Minimal promoter, natural 5¡¯ untranslated region (5¡¯UTR), natural intronÀ¸·Î ±¸¼ºµÈ native promoter
Minimal promoter, ´Ù¸¥ originÀ¸·ÎºÎÅÍ ¾ò¾îÁø 5¡¯UTR, enhanser·Î ±¸¼ºµÈ composite promoter°¡ ÀÖÀ½
* Ubiquitous and specific promoter
viral or cellular promoter·Î ³ÐÀº ¹üÀ§ÀÇ ¼¼Æ÷, Á¶Á÷, ¼¼Æ÷ ȸ·Î(cell cycle)¿¡¼ °·ÂÇÏ°Ô ¹ßÇöÇÏ´Â ubiquitous promoter
Specific typeÀÇ ¼¼Æ÷ ¶Ç´Â Á¶Á÷¿¡¼¸¸ ±×µéÀÇ È°¼ºÀ» ³ªÅ¸³»´Â cellular promoterÀÎ specific promoter
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º¸ °ü |
µ¿°á°ÇÁ¶µÈ transformed E.coli GT100 strainÀ¸·Î °ø±ÞµÊ, –20µµ |
À¯È¿±â°ü |
1³â |
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