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¡á¡á¡á Á¦Ç°Á¤º¸/½Ã¾à·ù/ Expression Vectors / INVIVOGEN/Multigenic Plasmid-pVITRO1

Ç° ¸ñ ¸í Multigenic Plasmid-pVITRO1  
¿ë    µµ Create gene combinations in a single plasmid        
±¸¼º¼ººÐ @ Æ÷À¯µ¿¹° ¼¼Æ÷¿¡¼­ 2°³ÀÇ À¯ÀüÀÚ¸¦ ¾ÈÁ¤ÀûÀ¸·Î co-expression
- 2°³ÀÇ °­·ÂÇÑ promoter
- SV40 & CMV enhancers
- ±¸Á¦¿ª ¹ÙÀÌ·¯½º (FMDV) IRES(internal ribosome entry site)
- °³¼±µÈ hygromycin selection
- 2°³ÀÇ multiple cloning sites ¶Ç´Â 2°³ÀÇ reporter genes
- Multigenic plasmid, 2°³ transcription units (TU)
- 2°³ÀÇ EF1 a promoters(origin : rat & mouse)
- Strong activity

@ pVITRO 1 backbone
First Transcription Unit     Second Transcription Unit
Promoter    Rat EF1¥á             Mouse EF1¥á
Enhancer   human CMV              SV40             
Gene        PLAP (MCS)             LacZ (MCS)
IRES        FMDV
Selection    HygroR        
º¸    °ü -20µµ        
À¯È¿±â°ü 1³â        

NO.CAT PRODUCT DESCRIPTION UNIT SIZE ÁÖ¹®¿äû
pvitro1-mcs @ pVITRO1-mcs(multiple cloning site) cDNAsÀÇ Æí¸®ÇÑ cloning, 2°³ÀÇ cDNA cloningÀ» À§ÇÑ MCS, ¸¹Àº restriction sites°¡ ÀÖ¾î ligation¿¡ ÀûÇÕ, MCS1 ¶Ç´Â MCS2¿¡ gene insertion 20ug °³
pvitro1-plaplacz @ pVITRO1-PLAP/LacZ ORF(open reading frame)À» ½±°Ô cloningÇÏ¿© control vector·Î ÀÌ¿ëÇÒ ¼ö ÀÖÀ½ 20ug °³

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